Hematopoietic stem cell aging

Current studies include:
• Functional studies on highly purified stem cells in young and old mice isolated from two different mouse strains: C57BL/6 and DBA/2
• Functional studies on stem cells isolated from mice having deficiencies in genes thought to be important for stem cell regulation

For additional information please contact:
• Prof. Gerald de Haan g.de.haan@umcg.nl

Homing and engraftment of (mobilized) stem cells

Current studies include:
• Studies to better identify cells with in vivo repopulating ability
• Methods to improve the functionality of mobilized blood stem cells

For additional information please contact:
• Prof. Gerald de Haan g.de.haan@umcg.nl

Hematopoietic stem cell expansion

Current studies include:
• Functional studies on stem cells cultured in the presence of various hematopoietic growth factors and/or inhibitors of differentiation

For additional information please contact:
• Prof. Gerald de Haan g.de.haan@umcg.nl

Epigenetic regulation in the hematopoietic system

Current studies include:
• Functional studies on stem cells in which one or more members of the Polycomb Group genes are overexpressed or downregulated
• Chromatin immunoprecipitation on members of the Polycomb group complex (complex compositions)

For additional information please contact:
• Dr. Leonid V. Bystrykh l.bystrykh@umcg.nl
• Prof. Gerald de Haan g.de.haan@umcg.nl

Clonal tracking in the hematopoietic system

Current studies include:
• Clonal tracking in vitro and in vivo, making use of multi-colored transgenic mice, multi-colored vectors and barcoded vectors
• Repeated retroviral mutagenesis approach aimed at identifying co-operating mutations in leukemogenesis

For additional information please contact:
• Dr. Leonid V. Bystrykh l.bystrykh@umcg.nl
• Prof. Gerald de Haan g.de.haan@umcg.nl

Systems biology – genome-wide approaches and data integration

Current studies include:
• Analyzing gene expression levels in different hematopoietic cell populations (isolated from genetically distinct mouse strains and of different ages)
Technologies: microarrays, quantitative PCR, deep sequencing
• Analyzing microRNA expression levels in different hematopoietic cell populations (isolated from genetically distinct mouse strains)
Technologies: microarrays, quantitative PCR
• Analyzing DNA methylation levels in different hematopoietic cell populations (isolated from genetically distinct mouse strains)
Technologies: promoter tiling arrays, bisulphite sequencing
• Integrating different data types and making these data accessible to the research community (www.hemdb.org)

For additional information please contact:
• Dr. Leonid V. Bystrykh l.bystrykh@umcg.nl
• Prof. Gerald de Haan g.de.haan@umcg.nl

Protection against radiation-induced damage to salivary glands by stem cell transplantation

This project is a collaborative effort of our Department with the group of Dr. Rob Coppes at the Department of Radiation and Stress Cell Biology. To ameliorate the late effects of radiation-induced damage to normal tissues such as salivary glands attempts are made to increase their capacity to regenerate. These studies currently involve attempts to culture tissue-specific stem cells. The cultured cells are phenotypically and functionally characterized using flow cytometry and cell culturing techniques as well as immunohistochemistry. For details please refer to the Dept of Radiation and Stress Cell Biology chapter.

For additional information please contact:
• Prof. Gerald de Haan g.de.haan@umcg.nl
• Dr. Rob Coppes r.p.coppes@umcg.nl

Evolution of leukemic clones

Current studies include:
• Functional studies on stem cells in which one or more transcriptional master regulators are overexpressed or downregulated
Technologies: Transplantation, lentiviral transduction, inducible systems
• Transcriptional network analysis on hematopoietic cells during leukemic evolution
Technologies: microarrays, next-generation sequencing, chromatin immunoprecipitation

For additional information please contact:
• Prof. Gerald de Haan g.de.haan@umcg.nl